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The ABI 7500 real-time PCR detection system was used to perform quantitative RT-PCR (qRT-PCR) following the manufacturers instructions and an SYBR green Premix Ex TaqTM kit (Takara)

Y., Zhang, Z., & Li, X

Finally, accelerating the transition of promising frontier strategiessuch as gene therapy and cell therapyfrom laboratory research to clinical validation and application is a critical step toward achieving the ultimate goals of neural repair and disease modification

It affects 20% to 44% of semaglutide users, depending on the study and dose level

Trial designs that implement inductionmaintenance sequences, co-primary mechanistic and clinical endpoints, longer follow-up for durability, and head-to-head comparisons under harmonized outcome sets are priorities